| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.023478 |
| Chromosome: | chromosome 13 |
| Location: | 5171069 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607300 | FAP352,MAPK5 | (1 of 1) K08293 - mitogen-activated protein kinase [EC:2.7.11.24] (E2.7.11.24); Flagellar Associated Protein 352 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCAACGGGGCGGCGGGCGGGCGGACCTCGACCACCAACACGCACTACA |
| Internal bar code: | GGTAGGCTCTTAAGTACGACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2524 |
| LEAP-Seq percent confirming: | 95.1219 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCTCCCTCTCATCCTCA |
| Suggested primer 2: | CCTGCTTCCCTTTCTCCCTG |