| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.023484 |
| Chromosome: | chromosome 13 |
| Location: | 2655834 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581300 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGGATCTCAGGCAGGGGCATCAGTCCAAGCCAGCAGCGCGCTGGTCGA |
| Internal bar code: | CTCCCGAGTAGTGTGCAGCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2613 |
| LEAP-Seq percent confirming: | 89.4737 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGATAAAGATGCGGATGC |
| Suggested primer 2: | GCAGATGTCTTTGGCGGTTG |