| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.023502 |
| Chromosome: | chromosome 1 |
| Location: | 6378092 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g045200 | (1 of 1) 5.3.99.6 - Allene-oxide cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGCGGCGGTGGGCGCGGCAGCCACAAGCGCGGCGGTTAGACCGCTATG |
| Internal bar code: | CGGAATCTATAGCGGCGTGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 761 |
| LEAP-Seq percent confirming: | 31.5789 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGCAACATAACCGCCACT |
| Suggested primer 2: | TACTCCCTTGCTTGCATGGG |