| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.023506 |
| Chromosome: | chromosome 16 |
| Location: | 432279 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693300 | 3'UTR | ||
| Cre16.g801869 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGCGGAGCACCCCGAAGATTCTACAGCTTGCTCTGGCATGTTTAATCC |
| Internal bar code: | GCCGTAGCCATGCCGTGCGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 780 |
| LEAP-Seq percent confirming: | 80.7692 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGCTGAGCTTCATGGCT |
| Suggested primer 2: | CTCGCACACTTTTTGCAGCT |