Insertion junction: CLIP2.023527_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.023527
Chromosome:chromosome 3
Location:137754
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g143927 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTTCTTTGCTTTGCCATGTCCTGCACCCAGCGGGGTTGTCTACTTGCA
Internal bar code:TGCCACGCTTCGCGCTAGGAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1456
LEAP-Seq percent confirming:78.9474
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTTTCCCTCGTAGCTGTT
Suggested primer 2:GTGCTACTGGGATCGAGAGC