Insertion junction: CLIP2.023553_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.023553
Chromosome:chromosome 8
Location:1665967
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g366050 (1 of 13) IPR000086//IPR015797 - NUDIX hydrolase domain // NUDIX hydrolase domain-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCACGGGTTCGTCCGTGACGCCCCGCCCTCCAGGACAACATCTGCCACG
Internal bar code:AGTGCCGTCGCTGTTCACCCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1917
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:65
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:GACCGCGACATACACAATGC
Suggested primer 2:GGCTCCTTTCTAGCACGTGT