| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.023559 |
| Chromosome: | chromosome 3 |
| Location: | 4584069 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGAGCTGTAGGCTTCGAAGAAATGTAAGCTAGTGTGAATGCTAGGCTT |
| Internal bar code: | CAATGTCGACGTTAGTTTCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3655 |
| LEAP-Seq percent confirming: | 97.619 |
| LEAP-Seq n confirming: | 82 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGCTAACATGTTCCTTCG |
| Suggested primer 2: | CAATGAGCTGCTGCGGATTC |