| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.023574 |
| Chromosome: | chromosome 6 |
| Location: | 3989250 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278208 | (1 of 1) PF04845 - PurA ssDNA and RNA-binding protein (PurA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCAGTTTAATGGCAGCAGCAGGTGGCTGCTGGTTGTATGGATGGGGCG |
| Internal bar code: | AAGGTGATATGCAAGCGGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4312 |
| LEAP-Seq percent confirming: | 98.6842 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGCGCATTCGTTCTTATT |
| Suggested primer 2: | ATAGGTCAAAAGCCCCCAGC |