Insertion junction: CLIP2.023611_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.023611
Chromosome:chromosome 11
Location:42989
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467526 (1 of 36) PF00583 - Acetyltransferase (GNAT) family (Acetyltransf_1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAAACAATGGAGTTTGCATCCCTCTGTGCTCCGGGGCGGAGTCGGATGC
Internal bar code:ATTGCAGTTTCTCGCACCGAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2411
LEAP-Seq percent confirming:98.0
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCGTTCATCCTTCACACA
Suggested primer 2:GGTGACAGCGAAGTGAGGAA