| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.023633 |
| Chromosome: | chromosome 13 |
| Location: | 3778374 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588900 | WEX,EXN15 | (1 of 7) IPR002562//IPR012337 - 3'-5' exonuclease domain // Ribonuclease H-like domain; putative Werner syndrome-like exonuclease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGAGGTGGCTGGCAGCGGGGGCGCACCCGAGCCCCGGCTGGTGGTA |
| Internal bar code: | TGTTCTGTGACCCCTTATGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2399 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATGCTTCCTTGACGTCT |
| Suggested primer 2: | TCCCCATCTCGTGAGGTCAT |