| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.023641 |
| Chromosome: | chromosome 12 |
| Location: | 2225237 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508853 | BPL2 | Biotin-protein ligase; (1 of 41) PF13920 - Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATTCAAGAACCGCCCTGCCTGCTGCGGGGATGTGGGGCCTGCAGGCAG |
| Internal bar code: | TCCTTACCCTGAATCATGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1609 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAGTGGATGTACGGTGG |
| Suggested primer 2: | TGCGTGTGTGATGCTGAGAT |