Insertion junction: CLIP2.023651_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.023651
Chromosome:chromosome 3
Location:7223823
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g207800 ADH5,CAD1 Cinnamyl-alcohol dehydrogenase; (1 of 2) 1.1.1.195 - Cinnamyl-alcohol dehydrogenase / CAD CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGGTCGCTGTGGCAGATGCCCGCATGCGTGATCTGAATGCGCACATCA
Internal bar code:GTATTATCGAAATACTCCGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1324
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTACAATCGTCGAACGCTGC
Suggested primer 2:TCTCATTCCCCCTCCCGAAT