| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.023654 |
| Chromosome: | chromosome 12 |
| Location: | 9097491 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCGCTTACCGCCAGCGCTTGGCGCGAATCTTCCGACATTGAAACTGC |
| Internal bar code: | GGTACGGGAGGGCGTGGCCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2919 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAGGGCAGAGGTAGCAA |
| Suggested primer 2: | TGTTGCCGTACAGGTGTACC |