| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.023704 |
| Chromosome: | chromosome 10 |
| Location: | 5514135 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g458050 | BCA3 | Branched chain amino acid aminotransferase; (1 of 1) 2.6.1.21//2.6.1.42 - D-amino-acid transaminase / D-aspartic aminotransferase // Branched-chain-amino-acid transaminase / Transaminase B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCCGCTCCCTCGGCACCGGCGAGGCCTCCTGTGTTGCGATGAATTGC |
| Internal bar code: | AGGTCGGGGATCAAGCCCATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4099 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCCTGGCTCTGAGTTGA |
| Suggested primer 2: | CTCACTTGCGCACGATGAAG |