Insertion junction: CLIP2.023715_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.023715
Chromosome:chromosome 9
Location:55518
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g800964 (1 of 31) PF03732 - Retrotransposon gag protein (Retrotrans_gag) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTAGAGGTGAAAGTAGACGTGGCGCGAGATTGCGGCTGCGGCTTAGAG
Internal bar code:TATGCTATTTATGGGACTCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2781
LEAP-Seq percent confirming:87.1795
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:TTCTCCCTCTCTCTCCAGCC
Suggested primer 2:TGCGCTAACTCGAACAGGTT