| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.023729 |
| Chromosome: | chromosome 12 |
| Location: | 8857395 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546450 | (1 of 1) PTHR12472//PTHR12472:SF0 - RAB3-GAP REGULATORY DOMAIN // RAB3 GTPASE-ACTIVATING PROTEIN NON-CATALYTIC SUBUNIT | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTAAAGCTCGGGAAGTACGGGACACACGCAGAGTGCTGGGCCACGCGC |
| Internal bar code: | CGAGCGCTCTCGAGAATTATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4926 |
| LEAP-Seq percent confirming: | 34.2593 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 71 |
| LEAP-Seq n unique pos: | 108 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGACGCCCTGCATCCATA |
| Suggested primer 2: | CACGTCATTGCGGTGATGTC |