Insertion junction: CLIP2.023765_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.023765
Chromosome:chromosome 3
Location:5085369
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g180250 IPS1,INO1 (1 of 1) 5.5.1.4 - Inositol-3-phosphate synthase / Myo-inositol-1-phosphate synthase; Myo-inositol-1-phosphate synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGCGCGGTAAAGGACTCCCGAGGCCGAGGCAACTTACTGCTTGTTGGC
Internal bar code:CTTTTATGAGGTGGTGGCTAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2436
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:58
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:58

Suggested primers for confirmation by PCR

Suggested primer 1:CCTACGACTATGCGTCCACC
Suggested primer 2:CGAAGTCGGGGTCGTAGATG