Insertion junction: CLIP2.023778_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.023778
Chromosome:chromosome 12
Location:5349594
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g528850 DLC3,ODA-LC3,DLX1 Flagellar Outer Arm Dynein Light Chain 3, thioredoxin; (1 of 7) 3.5.1.52 - Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase / N-oligosaccharide glycopeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGTACAGGCACCAATGACTCCATCCCGTTCCGTGCAAGGACCCGGGA
Internal bar code:ACTCAAACTTTCTGGATAGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2396
LEAP-Seq percent confirming:96.6102
LEAP-Seq n confirming:57
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGTGTTTGACGACCAACC
Suggested primer 2:TAGCTGCTCACTTCTTGCCC