Insertion junction: CLIP2.023786_4

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.023786
Chromosome:chromosome 10
Location:1097159
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g801112 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCATGTCCCTCCAGCAACCACTCTGCCATCAACGATATCCGACTGTCGC
Internal bar code:CTTTATATTTCGGTGTTTAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:583
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCCTGTCGGTTGAGGAAG
Suggested primer 2:CCGGTACAGCCCAATCCTTT