| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.023801 |
| Chromosome: | chromosome 6 |
| Location: | 1478820 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260500 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAACCAAATTCACATACGACGACTTCACGCCGCCAGACAGCGACCTGT |
| Internal bar code: | AATCAGCGTAAAGCGGATGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1529 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGTCTGAGTCCCGGAGAA |
| Suggested primer 2: | GCATGCGCCTATGGAGTTTG |