Insertion junction: CLIP2.023813_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.023813
Chromosome:chromosome 14
Location:1150265
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g615050 (1 of 1) 1.3.1.38//1.3.1.93 - Trans-2-enoyl-CoA reductase (NADPH) // Very-long-chain enoyl-CoA reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCGCACCCACCAGAAGTTGCCGAGCTGCGCGAGCACCGCCGCGGCCAG
Internal bar code:GAATCGACCATCGTTTTGAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:190
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGTAGGTCAGCAGGAGAG
Suggested primer 2:ATTCCCCCTCTCCAGGTCTC