Insertion junction: CLIP2.023816_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.023816
Chromosome:chromosome 6
Location:1434875
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g259950 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACGACGAGGTCCCGGGTGTGAAGGGCGCGGCGGCGGCGCGGGTGGTGA
Internal bar code:TGTTCGGTAAACGATTACCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1245
LEAP-Seq percent confirming:95.6522
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:ACATGTCCTGAAGCCGAGTG
Suggested primer 2:CATGCCATGTCGTGCTGATG