Insertion junction: CLIP2.023825_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.023825
Chromosome:chromosome 2
Location:6735732
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g387615 (1 of 3) PF10497 - Zinc-finger domain of monoamine-oxidase A repressor R1 (zf-4CXXC_R1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCATTGCGTGAGTGAATGTGCGGGCGCCGATGAGCGCCTCTTCTCGCG
Internal bar code:AAGTACTAATGTAGAGCCTTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2597
LEAP-Seq percent confirming:98.0392
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:GGCCTCCTTTGCCTTCATCT
Suggested primer 2:TTTCTGCCGCCAAAAGAAGC