Insertion junction: CLIP2.023834_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.023834
Chromosome:chromosome 6
Location:7631291
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g302000 MFP31,MFP1 Major facilitator superfamily transporter; (1 of 2) PF05977 - Transmembrane secretion effector (MFS_3) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCTCGATGGGGGGTAGCTTGTACTTGTACTGGCGCATACACCATGGT
Internal bar code:CCACCGAGAGACTTGTATTCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3047
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CATGAACTCGCTCATGCACG
Suggested primer 2:CTCTGGGCTTCATCCTAGCG