Insertion junction: CLIP2.023858_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.023858
Chromosome:chromosome 4
Location:2842150
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g224200 (1 of 2) PTHR18841//PTHR18841:SF0 - VITELLINE MEMBRANE OUTER LAYER PROTEIN I-RELATED // PROTEIN C08G5.3, ISOFORM A-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCATCATAAAGGCAGAGTAAACACAAGACTCACGTAGACGTAGTTGAG
Internal bar code:TTGCTGAAAAATGGTCTGGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1752
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CACATCCAGGGGACGAAACA
Suggested primer 2:CTGGGCAGCTTGGGTTTAGA