Insertion junction: CLIP2.023869_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.023869
Chromosome:chromosome 10
Location:4535965
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g451400 NIT4,CNX1B,CNX1E (1 of 1) 2.10.1.1//2.7.7.75 - Molybdopterin molybdotransferase // Molybdopterin adenylyltransferase; Molybdenum cofactor biosynthesis protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGTCTGCTTGGGGTATGGGCGTCAGCTGCACCCTATGGGACCTCGCCA
Internal bar code:TCCTAGGGAAGGTCGCGCGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:4396
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:CCTACATCACCACAGGCAGG
Suggested primer 2:TTCTGGTGTGCTGGTGGTTT