Insertion junction: CLIP2.023901_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.023901
Chromosome:chromosome 12
Location:9703663
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g555803 NDA6 Type-II NADH dehydrogenase; (1 of 5) 1.6.5.9 - NADH:ubiquinone reductase (non-electrogenic) / Ubiquinone reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCCTTTTCATTAACCCACAAACACCCACGCACATGCCTCATCTCCCAC
Internal bar code:CATGGGGAATCGTTGGTTTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1467
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCATCACACAAACACACG
Suggested primer 2:GTATCTACAACCCCCACGCC