| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.023929 |
| Chromosome: | chromosome 7 |
| Location: | 3702378 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g337750 | EBM5 | (1 of 3) PTHR31451//PTHR31451:SF1 - FAMILY NOT NAMED // MANNAN ENDO-1,4-BETA-MANNOSIDASE 2-RELATED; Mannan endo-1%252C4-beta-mannosidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACAGATGTCACACACACACACACACACACACACACACACACACACACAC |
| Internal bar code: | GTGTTCGTGGTGGGTCACTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1351 |
| LEAP-Seq percent confirming: | 59.375 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCATACCGCCTGTGTCT |
| Suggested primer 2: | GCTGGAGCTACTGTGTGTGT |