Insertion junction: CLIP2.023946_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.023946
Chromosome:chromosome 11
Location:2138841
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467743 (1 of 3) PF04434 - SWIM zinc finger (SWIM) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCCGTGAAACATGTGCACGTGCGCCGGGGCGCATCATGGCTGCAATAA
Internal bar code:TTAGGGACGTACGGAGAATTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2370
LEAP-Seq percent confirming:96.6667
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAGTACGGGCTGTTGGAT
Suggested primer 2:AACTTCGGGAGCTTGAGGTG