| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.023951 |
| Chromosome: | chromosome 14 |
| Location: | 3010545 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g627650 | FAP392 | Flagellar Associated Protein 392 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTTCGACGTGGCGGGGGCGCTGGCGTGGGCGCGGGAGATGGCGGCTGC |
| Internal bar code: | GATTGATATTTTGTCATTGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3467 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCATCTAGGTGTGGCTTT |
| Suggested primer 2: | CCTTCCGCCTGAACAAGCTA |