Insertion junction: CLIP2.023976_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.023976
Chromosome:chromosome 11
Location:3190142
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g475950 HLM16 putative N-methyltransferase; (1 of 5) IPR001214//IPR003616 - SET domain // Post-SET domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAAAGAAAGGAGAGGGGTGGGCGGGCGGGCAACAAAAGAGAGCGCCAGC
Internal bar code:TTTGGGTAGCAACGGATTCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3536
LEAP-Seq percent confirming:14.2857
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:TGAGTGCGTGTGCTATGTGT
Suggested primer 2:GTGGGTGGGTGGGTGTTAAA