Insertion junction: CLIP2.023980_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.023980
Chromosome:chromosome 12
Location:2706353
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g505050 (1 of 13) PF00646 - F-box domain (F-box) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGTGCTGACGACTCCCAACTCCACAATTACATCGTTGCACGCTGGCAGA
Internal bar code:TGTTTCTTCAGACGCATGTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3094
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:TCCACCCATGCTCTCCAAAC
Suggested primer 2:CAAGCACACGGGTCTGTTTG