Insertion junction: CLIP2.023981_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.023981
Chromosome:chromosome 1
Location:2032522
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g011150 (1 of 2) IPR000104//IPR011598 - Antifreeze protein, type I // Myc-type, basic helix-loop-helix (bHLH) domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGTCTCGGCGGCGCCAGCAGGGGTACTACTGCTGAGCGCGTAGGTGTG
Internal bar code:GGTCATAGGTGTTCTTACAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:135
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGACGACGACATTGGAAACG
Suggested primer 2:AGTGTCTCGTTGTCCTGCTG