Insertion junction: CLIP2.023998_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.023998
Chromosome:chromosome 4
Location:1806446
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre04.g211600 snRK1,CKIN1,SNF1,SNRK1A (1 of 1) PTHR24343//PTHR24343:SF183 - SERINE/THREONINE KINASE // SUBFAMILY NOT NAMED; snRK1 family in Chlamydomonas, subgroup 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCCCACCCCACCACATCCCACATCCAACCCCACGTCACCCTACCCCCA
Internal bar code:GGGCTCGGGGTACTTGTTAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:102
LEAP-Seq percent confirming:21.4286
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTGGTTCCGAGCTCTATC
Suggested primer 2:CGCGATCTTGAGGCTCATCT