| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.024003 |
| Chromosome: | chromosome 7 |
| Location: | 1722842 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325716 | DIV146,DNL1,LIG1 | DNA ligase 1; (1 of 1) K10747 - DNA ligase 1 (LIG1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCGGTATGGCAGCCTGATGTGTGTGCAGGCGGTGCGGTAGGTGGTGC |
| Internal bar code: | TTCGACTGTCTAACATAAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 230 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGTGAACTTCTCCAGCAC |
| Suggested primer 2: | AACGAGGCCAGCATCAAGAA |