Insertion junction: CLIP2.024015_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.024015
Chromosome:chromosome 8
Location:2430123
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g371957 RAPTOR1,RAPTOR Regulatory-associated protein of TOR; (1 of 1) K07204 - regulatory associated protein of mTOR (RAPTOR) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACCCGCGCGTTACGCATGCCTAAACTCTACGCACAAGCACGTCGTCGT
Internal bar code:TCCCAATGATCCAATTGGTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:133
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCCATAGGCTGTAGCACCAG
Suggested primer 2:GGAGGAAGAGAGGTAGGGCA