Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.024030 |
Chromosome: | chromosome 17 |
Location: | 6131061 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g802133 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTTGGCTGCCGCCACGGCTCTCCACCGCCTCTTCTTCACGCGCCACGA |
Internal bar code: | GAAAGTAGTTGACAGGGATAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2072 |
LEAP-Seq percent confirming: | 90.4762 |
LEAP-Seq n confirming: | 38 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACGCCCACAAATGCAAACT |
Suggested primer 2: | AGACGAGCCACACCCAAAAA |