| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.024047 |
| Chromosome: | chromosome 6 |
| Location: | 3048988 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274800 | HTA4,HTA26 | Histone H2A; (1 of 30) K11251 - histone H2A (H2A) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGGCAAGAAGGCCGTGAGCCGCAGCGCCAAGGCCGGCCTGCAGTTCC |
| Internal bar code: | CAGTGTTATAGCGTCAGAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3036 |
| LEAP-Seq percent confirming: | 94.1667 |
| LEAP-Seq n confirming: | 113 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 120 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAATACCGGAGCCAGGTTT |
| Suggested primer 2: | GCTTAGCCCGAGGTGAACTT |