Insertion junction: CLIP2.024080_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.024080
Chromosome:chromosome 10
Location:5040105
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g801174 (1 of 19) 1.14.14.1 - Unspecific monooxygenase / Xenobiotic monooxygenase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTTGAGGTTGTGTGCTCCGCGCCAAGCGAGGACAATGCGCCAATGCCTC
Internal bar code:ACCATATCACATTGGTGCACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1674
LEAP-Seq percent confirming:94.7368
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TCGAAGGAAAGGCATGTGCT
Suggested primer 2:ATGCATTTGGGTGAGGGAGG