| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.024096 |
| Chromosome: | chromosome 14 |
| Location: | 3587233 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g801659 | intron | ||
| lncRNA_TCONS_00105207 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAGTATTGATAACGCGAGGTGTTGCGGCGCACTAGAGACTCCTGGAGA |
| Internal bar code: | TCCCACTGATGCATAGCTTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1220 |
| LEAP-Seq percent confirming: | 47.0588 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCGGAGACTGAAATTGG |
| Suggested primer 2: | CCGTTCTCATTCTTGGGCCT |