| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.024115 |
| Chromosome: | chromosome 7 |
| Location: | 5594678 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g800857 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGAAGTGCTCGCAGCGACTACCTCAGTTGGCTTGGCCTTTGTGGTGAC |
| Internal bar code: | ATGCTAGGGCCTTAGACGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2219 |
| LEAP-Seq percent confirming: | 61.194 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCAAGAAAGCGTTCCGT |
| Suggested primer 2: | GAGTCGGGCATGAGATGAGG |