| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.024125 |
| Chromosome: | chromosome 2 |
| Location: | 7597568 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386912 | DGTT2 | Diacylglycerol acyltransferase, DGAT Type 2; (1 of 4) K14457 - 2-acylglycerol O-acyltransferase 2 (MOGAT2, MGAT2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACTAGTAAACCTGGGTACCAACATAGATCCAGGCCAAGTCCCATGCAC |
| Internal bar code: | ACGTGTAAAGTAGCTCTTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2264 |
| LEAP-Seq percent confirming: | 96.5116 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCTGGGAATCCTTGTGG |
| Suggested primer 2: | AGCCGGGAAACATGATCTGG |