| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.024147 |
| Chromosome: | chromosome 13 |
| Location: | 1096950 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g569150 | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGGATGGCCTCCCCGGAGCGGTTGAGCACGGACACGTGGCCAACCAC |
| Internal bar code: | TTTGGAGGTTTGTAAGACATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3411 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 140 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 145 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCCTCAGATGGTGCCAC |
| Suggested primer 2: | TATAACTCCTCGACGCGCAC |