Insertion junction: CLIP2.024154_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.024154
Chromosome:chromosome 7
Location:5991544
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g354600 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCACCGACACAAGCGACCTGGCCCCCTTGCTGCCGCCGCCACCAACCG
Internal bar code:TACTAGATTTCTCTAGTCCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1137
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ACACCTTCATCGACCTGCTG
Suggested primer 2:TATTGTGACGGGCGAGCTTT