Insertion junction: CLIP2.024176_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.024176
Chromosome:chromosome 2
Location:2917601
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095150 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGACAGCGTCACCACCGCGAAGTATGGGTTGTTGAAGTTGGTCCAGAA
Internal bar code:TGTGGTTTGGCGCAAAGTGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1056
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTCTGGGTGGACCTGTTC
Suggested primer 2:CCCACGGTGATGACCATGAA