| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.024178 |
| Chromosome: | chromosome 6 |
| Location: | 2361822 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g268100 | HTB10 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACAAGAAGAAGAAGAAGAGTGCCGTGGAGACCTACAAGCTGTACATC |
| Internal bar code: | TCGCACCGCCTAGAATTGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4831 |
| LEAP-Seq percent confirming: | 89.8305 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTAAAGGCTGGCAGTGAG |
| Suggested primer 2: | TTGCCCTTCTTCAGGTAGCG |