Insertion junction: CLIP2.024183_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.024183
Chromosome:chromosome 2
Location:2797602
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g094300 FUO1 (1 of 1) 1.5.5.1 - Electron-transferring-flavoprotein dehydrogenase / ETF-ubiquinone oxidoreductase; Electron transfer flavoprotein-ubiquinone oxidoreductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGATTGTGGTGGTGATGCGTCCTCGTGCACAGGGACCTGTGGACATGTC
Internal bar code:GATCGATCACAGGTTCTCTGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2267
LEAP-Seq percent confirming:97.8261
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:GAACTCGCCTTCGTCCTTGA
Suggested primer 2:TACGACAACCCGCACATCAA