| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.024183 |
| Chromosome: | chromosome 2 |
| Location: | 2797605 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094300 | FUO1 | (1 of 1) 1.5.5.1 - Electron-transferring-flavoprotein dehydrogenase / ETF-ubiquinone oxidoreductase; Electron transfer flavoprotein-ubiquinone oxidoreductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACAACAATTACAAATTACATGCCTTAGTTGCGCTACCCATGTTAGTTG |
| Internal bar code: | GATCGATCACAGGTTCTCTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5553 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 114 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 114 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGACAACCCGCACATCAA |
| Suggested primer 2: | GAACTCGCCTTCGTCCTTGA |