| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.024210 |
| Chromosome: | chromosome 12 |
| Location: | 4981061 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524950 | ENGB,ENGB1 | Factor possbly involved in ribosomal 50S subunit assembly; (1 of 1) PTHR11649//PTHR11649:SF49 - MSS1/TRME-RELATED GTP-BINDING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATCTCGCCCTCCTCCTCGTCCTCGCCTTCCACGTCGTCCGCCCCGCCC |
| Internal bar code: | TGGTAATTTACGTACCTCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2740 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATCAGGCTCCTCGGATTC |
| Suggested primer 2: | GGTATCTGCTCACTCCGTCG |