| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.024212 |
| Chromosome: | chromosome 2 |
| Location: | 6679204 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387134 | (1 of 3) PF04379 - Protein of unknown function (DUF525) (DUF525) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCGCTCGCAAGGTGCAGCGCGACGCTGGCGACGCACTGAAACCCTGT |
| Internal bar code: | GTCGAGGGTTGGGCGAGCTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2595 |
| LEAP-Seq percent confirming: | 90.8257 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 109 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCACCTCGTAGGCTCCTT |
| Suggested primer 2: | CACGTATTCAGCATGCACCG |